Journal: PLoS ONE

Article Title: Free Access to a Running-Wheel Advances the Phase of Behavioral and Physiological Circadian Rhythms and Peripheral Molecular Clocks in Mice

doi: 10.1371/journal.pone.0116476

Figure Lengend Snippet: Plasma corticosterone (A), leptin (B) and ghrelin (C) concentrations at indicated times in mice housed under sedentary (SED; unfilled circles) conditions or given free access to running-wheels (RW; filled circle). Gray shading indicates dark period. Data are shown as means ± SEM ( n = 4–5). * P < 0.05 and ** P < 0.01, significant differences between SED and RW mice at corresponding Zeitgeber times (ZT).

Article Snippet: Plasma concentrations of corticosterone, leptin and ghrelin were measured using AssayMax Corticosterone ELISA kits (AssayPro LLC, St. Charles, Missouri, USA), Mouse Leptin ELISA kits (Morinaga Institute of Biological Science Inc., Kanagawa, Japan) and Desacyl-Ghrelin ELISA kits (Mitsubishi Chemical Medience Corporation, Tokyo, Japan), respectively.

Techniques: Clinical Proteomics

Journal: PLoS ONE

Article Title: Long-lived weight-reduced αMUPA mice show higher and longer maternal-dependent postnatal leptin surge

doi: 10.1371/journal.pone.0188658

Figure Lengend Snippet: Each time point represents the mean ± s.e.m. of 5 to 17 mice (see for details). Pups were sampled every four days, following four hours of chow deprivation (first experiment), and were weaned at day 24 (P24). (A) Postnatal circulating leptin level measured using ELISA kit (R&D Systems Inc.). Insert of A. Mean P4-P24 leptin level. Bars with different letters are significantly different ( P <0.05, based on two-ways ANOVA followed by a post-hoc test). (B) Leptin:FM ratio. *, P <0.05 by post hoc comparison following one-way ANOVA of all four mice groups.

Article Snippet: Leptin level in the serum, milk, and stomach content was measured using a mouse leptin ELISA kit (R&D Systems Inc.), as used previously for both strains [ , ].

Techniques: Enzyme-linked Immunosorbent Assay, Comparison

Journal: PLoS ONE

Article Title: Long-lived weight-reduced αMUPA mice show higher and longer maternal-dependent postnatal leptin surge

doi: 10.1371/journal.pone.0188658

Figure Lengend Snippet: Multiple regression of leptin on strain, gender, age, fat mass, and fat-free mass.

Article Snippet: Leptin level in the serum, milk, and stomach content was measured using a mouse leptin ELISA kit (R&D Systems Inc.), as used previously for both strains [ , ].

Techniques:

Journal: PLoS ONE

Article Title: Long-lived weight-reduced αMUPA mice show higher and longer maternal-dependent postnatal leptin surge

doi: 10.1371/journal.pone.0188658

Figure Lengend Snippet: Multiple regression of leptin on age, fat mass, strain, and gender, carried out separately for each age group.

Article Snippet: Leptin level in the serum, milk, and stomach content was measured using a mouse leptin ELISA kit (R&D Systems Inc.), as used previously for both strains [ , ].

Techniques:

Journal: PLoS ONE

Article Title: Long-lived weight-reduced αMUPA mice show higher and longer maternal-dependent postnatal leptin surge

doi: 10.1371/journal.pone.0188658

Figure Lengend Snippet: Each time point represents the mean ± s.e.m. of 6 to 22 mice, females only. Pups were sampled every four days, following four hours of chow deprivation (first and second experiments) and dam deprivation (second experiment only). Leptin and corticosterone levels were measured using ELISA kits (R&D Systems Inc.). (A and B) Postnatal circulating leptin level of dam-deprived and non-deprived female WT (left) and αMUPA (right) mice. (C) Postnatal circulating leptin levels of dam-deprived female αMUPA and WT mice. (D) Postnatal body weight growth curves of dam-deprived and non-deprived female WT mice. (E) Postnatal body weight growth curves of dam-deprived and non-deprived female αMUPA mice. (F) Postnatal body weight growth curves of dam-deprived female αMUPA and WT mice. (G) Mean P4-P24 leptin level of dam-deprived and non-deprived female αMUPA and WT mice. (H) Corticosterone P12 level of dam-deprived and non-deprived female αMUPA and WT mice. Bars with different letters are significantly different ( P <0.05, based on two-ways ANOVA followed by a post-hoc test). *, P <0.05 by post hoc comparison following one-way ANOVA of all four mice groups.

Article Snippet: Leptin level in the serum, milk, and stomach content was measured using a mouse leptin ELISA kit (R&D Systems Inc.), as used previously for both strains [ , ].

Techniques: Enzyme-linked Immunosorbent Assay, Comparison

Journal: PLoS ONE

Article Title: Long-lived weight-reduced αMUPA mice show higher and longer maternal-dependent postnatal leptin surge

doi: 10.1371/journal.pone.0188658

Figure Lengend Snippet: Bars represent the mean ± s.e.m. of 6 dams per strain (A) and their female pups (B), one female pup per dam, all sampled at P12. Dams' serum and milk samples (A) were obtained 4 hours after their separation from their pups. Milk secretion was encouraged by subcutaneous oxytocin injection (2 IU/kg) administered 15 min before sampling. Pups' serum and stomach content (B) were sampled 30 min after they were reunited with their dams. Leptin level was measured using ELISA kit (R&D Systems Inc.). *, P<0.05 between strains, by Mann-Whitney non-parametric test. #, P<0.05 within strains, by Wilcoxon non-parametric Test.

Article Snippet: Leptin level in the serum, milk, and stomach content was measured using a mouse leptin ELISA kit (R&D Systems Inc.), as used previously for both strains [ , ].

Techniques: Injection, Sampling, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: International Journal of Biological Sciences

Article Title: Chronic Stress Induces Hepatic Steatosis via Brain-Hepatic Sympathetic Axis Mediated Catecholamine Resistance

doi: 10.7150/ijbs.126058

Figure Lengend Snippet: Blockade of adipose sympathetic nerve does not prevent chronic stress-induced impairment of hepatic lipid homeostasis. (A-D) FFA, leptin, adiponectin, resistin levels in serum, n = 10 per group. (E) Experimental scheme. (F) Body weight, n= 5:6. (G) Food intake, n = 5:6. (H-K) Serum ALT, AST, TG and TC concentrations, n = 5:6. (L-O) FFA, leptin, adiponectin, resistin levels in serum, n = 5:6. (P) Liver weight and liver anatomy, n = 5:6. (Q) Liver TG, n = 5:6. (R) H&E staining (scale bars, 20 μm) and Oil Red O staining (scale bars, 20 μm) of liver sections, n = 5:6. (S) Relative mRNA levels in liver, n = 5:6. The data are presented as mean ± SEM. * P < 0.05. Unpaired two-tailed Student's t -test and one-way ANOVA were used for statistical analysis.

Article Snippet: Serum leptin (E-EL-M3008), adiponectin (E-EL-M0002), resistin (E-EL-M3056), norepinephrine (NE, E-EL-0047), and hepatic cyclic adenosine monophosphate (cAMP, E-EL-0056) in liver extracts were measured using enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer's intructions (Elabscience, Wuhan, China).

Techniques: Staining, Two Tailed Test

Journal: Heliyon

Article Title: Improved leptin sensitivity and increased soluble leptin receptor concentrations may underlie the additive effects of combining PYY [ [1] , [2] , [3] , [4] , [5] , [6] , [7] , [8] , [9] , [10] , [11] , [12] , [13] , [14] , [15] , [16] , [17] , [18] , [19] , [20] , [21] , [22] , [23] , [24] , [25] , [26] , [27] , [28] , [29] , [30] , [31] , [32] , [33] , [34] ] and exendin-4 on body weight lowering in diet-induced obese mice

doi: 10.1016/j.heliyon.2024.e32009

Figure Lengend Snippet: PYY(3 – 36) and Ex4 in combination increases leptin sensitivity and increases the concentration of circulating soluble leptin receptor. A-D) Accumulated food intake (kcal) and body weight change (g) 4h (A, B) and 20h (C, D) after leptin injection(5 mg/kg) at treatment day 10.E) Plasma leptin concentration (pM) at day 15, F: Plasma concentration of soluble leptin receptor (SLR) at day 15, and G: Hepatic Lepra expression at day 15. Data are expressed as means ± SEM, n = 12. Statistical significance was assessed by one-way ANOVA (E) or by two-way ANOVA followed (F and G). In both cases, ANOVA test's were followed Tukey's multiple comparisons test. In case of A-D, test of significance was restricted to comparing within group effects (vehicle and leptin treatment). *p < 0.05, **p < 0.01, and ***p < 0.001.

Article Snippet: Soluble leptin receptor was analysed by a mouse leptin receptor picokine ELISA kit (BOSTER biological technology, Cat # EK0440).

Techniques: Concentration Assay, Injection, Clinical Proteomics, Expressing